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Organisation : Krishna University, Machilipatnam
Announcement : Syllabus
Entrance Exam : KRUCET-2017 Krishna University Common Entrance Test
KRUCET Syllabus :
The Entrance Test will have 100 objective multiple choice type questions. The question paper generally will consist of three sections viz., Sections A and B with 30 question each and Section C with 40 questions.
Related : KRUCET 2017 Krishna University Common Entrance Test : www.indianin.in/4906.html
In case of tie priority in ranking is given on the basis of marks obtained in Section C followed by Section B. For some subjects the number of questions may be less.
Test-Wise Syllabus :
Test No.01 M.Sc., Biochemistry
Section A :
Structure and functions of liver, kidney, Composition of blood, blood coagulation, Digestion and absorption of proximate principles, Biological value of proteins, protein malnutrition disorders, Chemistry and physiological role of vitamins, Role of minerals in living systems, Structure and physiological roles of hormones, Basic features of immune response, Lymphoid system, T and B lymphocytes, Cellular and humoral immunity, Classes and structure of immunoglobulin, RIA, ELISA, Vaccines.
Bacteria, Viruses, Organisation of genome in prokaryotes and eukaryotes, DNA replication, Biosynthesis of RNA, Protein synthesis, Genetic code, Inhibitors of DNA replication, transcription and translation, Basic concepts of regulation of gene expression, principles and applications of recombinant DNA technology.
Principles of Enzymology, Bioenergetics, Biological Oxidations, Metabolism of carbohydrates, lipids, proteins, amino acids and nucleic acids
Section C: Classification, chemistry and physico-chemical properties of amino acids, proteins, nucleic acids, carbohydrates, lipids, porphyrins, PH and Buffers, Biomembranes–composition and organisation, Basic principles of biochemical separation methods–paper, thin layer,ion-exchange,affinity chromatography, gel filtration,Centrifugation,Electrophoresis–paper, polyacrylamide,agarose gels.
Basic principles of Colorimetry and spectrophotometry.Radio isotopes and their use in Biochemistry.
TEST NO. 02: M.Sc., Biotechnology
Physical Sciences and Mathematics –Atomic number and atomic weight. Electronic configurations, Electronic theory of valency. Oxidation states, Oxidation numbers. Common redox and ionic reactions. Nuclear chemistry, composition of nucleus, binding energy and packing fraction, Nuclear reaction.
Uses of radioisotopes, Electron displacements, bond formation and fission and reaction intermediates, Nucleophiles and electrophiles. Alkanes, alkenes and alkynes, alkyl halides, alcohols, aldehydes, ketones, acids, esters and amino preparations, properties and reactions, Benzene-aromaticity.
Dilute solutions – Osmotic pressure, lowering of vapour pressure, depression of freezing point and elevation of boiling point. Molecular weight determination in solution, association and dissociation of solutes.
Chemical kinetics-Molecularity and order of a reaction – first order, second order and rate equation. Electrochemistry, laws of electrolytes, conductivity of an electrolyte, hydrolysis of salts, hydrogen ion concentration, buffer solutions and acid-base indicators.
Role of metals in biological systems, sodium, potassium, iron, zinc and cobalt, toxic metals. Arsenic, mercury, lead, inorganic and organic pollutants – their reactivity and effect of some pollutants on human and plant life.Application of mathematics in biology. General introduction to computers.
Life Sciences: Cell structure and function, Cell organelles, Cell division. Genetics, laws of inheritance, Linkage, Crossing over, Polyploidy, Hybrid vigour and Sex determination.
Structure and replication of DNA, genetic recombination, transcription and post transcriptional modifications, Concept of genetic code, RNA and ribosomes – their function and role in protein synthesis. Gene cloning, application of gene cloning in medicine, Gene Mapping, Mutations.
Cell and its biochemical organization – Structure, chemistry, properties and metabolism of carbohydrates, proteins, fats and vitamins Concept of basic metabolism, Role of enzymes in Metabolism, Enzyme kinetics, inhibition, and Regulation.
Microbiology – Classification and identification of bacteria, viruses and other microorganisms, structure and isolation, culture methods, role of microbes in carbon, nitrogen and sulphur cycles in nature. Immunology – Organism immunity, immunity to infectious diseases and vaccines.
Section C :
Principles of Biotechnology:
Principles and applications of centrifugation, Paper and Thin layer chromatography, paper electrophoresis, agarose gel electrophoresis and their applications. Principle and applications of UV- Visible spectroscopy. Microscopic techniques–Light and electron microscopy.
Fermentation – Introduction, types of fermentation processes (batch, fed-batch, continuous), types of fermenters (Stirred tank & Air-Lift Fermenters), feed culture preparation, inoculum preparation and product yield.
Product Recovery Techniques (Biomass separation, filtration, centrifugation, membrane separation), Product purification techniques (Dialysis, reverse osmosis, organic solvent extraction). Production of alcoholic beverages (Beer and wine), antibiotics (Penicillin), organic acids (Citric acid), amino acids (Glutamic acid).
Single cell proteins and Probiotics. Media used for plant tissue culture, different components of media, sterilization of media, Callus culture, Meristem culture, Somatic embryogenesis, Synthetic seeds, transgenic plants and applications.
Serum and serum free media used for maintenance of animal cell lines, Sterilization of media, Maintenance of primary cell line and secondary cell lines, transgenic animals and their applications.
Definition and scope of environmental biotechnology, Municipal solid waste disposal, Microbiological treatment of municipal sewage and industrial effluents, Waste water treatment system (Aerobic, anaerobic and activated sludge process), Bioremediation, Biodegradation.